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首次采用同时蒸馏萃取法(SDE)提取了人工培育的长座线虫草子实体及摇瓶菌丝体的挥发性成分,并用气相色谱质谱联用仪(GC-MS)进行了分析。结果表明,不同培养方式对长座线虫草挥发性物质的数目和含量有较大影响,对挥发性成分的类型影响较大,液体摇瓶菌丝体挥发性物质种类主要有:酮类、醇类、烷烃类、烯烃类、醌类和醚类化合物;子实体中挥发性物质种类主要有:醇类、醛类和酚类。液体摇瓶菌丝体鉴定出20种挥发性物质,以1-辛烯-3-醇(37.70%)为主,人工培养子实体鉴定出6种挥发性物质,以1-辛烯-3-醇(51.93%)和2,6-二叔丁基对甲苯酚(38.37%)为主。2种人工培养物挥发性物质的共有成分为共有成分1-辛烯-3-醇、2,6-二叔丁基对甲苯酚在菌丝体中占51.66%,在子实体的挥发性成分中占90.30%。
The volatile components of artificial Cordyceps militaris and shake flask mycelia were extracted by simultaneous distillation extraction (SDE) and analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that different culture methods had a greater impact on the number and content of volatile substances in Cordyceps sinensis, and had a greater impact on the types of volatile components. The main volatile liquid species in liquid flask were ketone, alcohol Alkanes, olefins, quinones and ethers; the main types of volatile substances in the fruiting bodies are: alcohols, aldehydes and phenols. Twenty kinds of volatile substances were identified in mycelium of liquid shake flask, and 1-octen-3-ol (37.70%) was the main material. Six kinds of volatile substances were identified in artificial culture medium. Alcohol (51.93%) and 2,6-di-tert-butyl-p-cresol (38.37%) dominated. The common components of the two kinds of artificial culture volatile substances were the common component 1-octen-3-ol, and the 2,6-di-tert-butyl p-cresol accounted for 51.66% of the mycelium. The volatile components In 90.30%.