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目的观察BALB/c小鼠感染日本血吸虫后18周内血清中可溶性虫卵抗原(SEA)特异性IgG、IgM抗体水平的动态变化。方法尾蚴感染BALB/c小鼠,感染2周后开始每周采血并分离血清,以SEA为抗原,通过ELISA测定不同感染时间血清IgG、IgM值;通过免疫印迹法检测不同感染时间小鼠血清中SEA特异性IgG、IgM抗体条带。结果ELISA检测结果表明感染小鼠血清IgG水平在感染5、6、9、11周上升明显;IgM在感染5周和9周时上升明显。免疫印迹试验结果表明感染血吸虫4周后,小鼠血清中开始出现140、180、210 kDa蛋白特异性IgG抗体;感染5周后出现43、50 kDa强反应IgG特异性抗体带;感染6周后在60～130 kDa处出现IgG特异性弥散带;感染9周后出现38、73 kDa蛋白特异性IgG条带,其中38 kDa条带较弱,随感染时间延长,条带反应逐渐增强,直至感染18周;感染11周后新增26、32、35、80 kDa特异性IgG条带,并且在12周后增强。感染3周后出现100、140、180 kDa IgM特异性反应条带,感染9周后出现73 kDa特异性IgM强反应条带及38、43、50 kDa弱反应IgM抗体带,后3条蛋白条带随感染时间延长逐渐变强。结论SEA中不同组分抗原对感染宿主的免疫调节具有时间特异性,其中43、50、100、140、180 kDa具有早期诊断价值;73 kDa既可用于急性血吸虫病诊断,也可用于慢性血吸虫病诊断;28、32、35、38、80 kDa抗原既可作为慢性血吸虫感染的优势抗原,同时也可能具有一定的抗血吸虫感染作用,可作为疫苗开发的候选抗原。
Objective To observe the dynamic changes of serum specific IgA and IgG antibodies in BALB / c mice infected with Schistosoma japonicum within 18 weeks. Methods BALB / c mice were infected with cercariae. Two weeks after infection, blood samples were collected weekly and sera were separated. Serum IgG and IgM were measured by ELISA at different times of infection with SEA as antigen. Serum SEA-specific IgG, IgM antibody bands. Results The results of ELISA showed that the level of serum IgG in infected mice increased significantly at 5, 6, 9 and 11 weeks of infection, while IgM increased at 5 and 9 weeks of infection. Immunofluorescence results showed that four weeks after infection, 140,180,210 kDa protein-specific IgG antibodies began to appear in the sera of mice, and 43,50 kDa IgG-specific antibody bands appeared after 5 weeks of infection. After 6 weeks of infection At 60 ~ 130 kDa IgG-specific diffusive bands appeared; after 9 weeks of infection, 38,73 kDa protein-specific IgG bands appeared, of which 38 kDa bands were weaker. With prolonged infection, the band reaction gradually increased until the infection 18 weeks; 26, 32, 35, 80 kDa specific IgG bands were added after 11 weeks of infection, and enhanced after 12 weeks. After 3 weeks of infection, 100,140,180 kDa IgM-specific reaction bands appeared. After infection for 9 weeks, 73 kDa specific IgM strong reaction bands and 38,43,50 kDa weakly reactive IgM antibody bands appeared, and the last 3 protein bands With the extension of infection time becomes stronger. Conclusion The immunoregulation of different antigenic components of SEA in infected hosts is time-specific, and 43, 50, 100, 140 and 180 kDa have early diagnostic value. The 73 kDa can be used for the diagnosis of acute schistosomiasis and chronic schistosomiasis Diagnosis; 28,32,35,38,80 kDa antigen can be used as the dominant antigen of chronic schistosome infection, but also may have some anti-schistosomiasis, can be used as a vaccine candidate antigen.