作者想对与抗Lud冶凝集素相关的抗原分子进行定位。该凝集素的特异性与能识别N-乙酰神经氨酸依赖抗原的抗-Lud抗体相一致。作者采用伴有加热洗脱步骤的免疫印迹方法来确定Lud抗原。先用SDS-聚丙烯酰氨凝胶电泳分离的红细胞膜蛋白转移到硝酸纤维膜上后,再与抗-Lud4℃保温孵育,最后与连接有抗人1g抗体的过氧化物酶保温孵育。抗Lud抗体与未经处理过的红细胞上的分子量约为43000道尔顿的蛋白质发生反应。神经氨酸酶对红细胞的修饰作用,导致了免疫过氧化物酶染色带的 The authors want to locate the antigen molecules associated with anti-Lud metalloproteinases. The specificity of the lectin is consistent with an anti-Lud antibody that recognizes N-acetylneuraminine-dependent antigens. The authors used immunoblotting with a warm-up step to determine the Lud antigen. First, the erythrocyte membrane protein separated by SDS-polyacrylamide gel electrophoresis was transferred onto a nitrocellulose membrane, incubated with anti-Lud4 ° C., incubated with peroxidase incubated with anti-human 1 g antibody. Anti-Lud antibodies react with proteins of approximately 43,000 daltons on untreated erythrocytes. Neuraminidase modification of red blood cells, leading to the immune peroxidase staining zone