本文报导用10%三氯醋酸(TCA)液既作血浆蛋白沉淀剂又作甲硝唑提取溶剂,在320nm处测定沉淀血浆蛋白后的10%TCA液与其碱液的吸收度差的差示分光光度法。本法操作简便,灵敏度高,重现性较好,可排除血浆中紫外吸收物质的干扰,符合临床药液监测及药代动力学研究的要求。根据测定动物体内血药浓度甲硝唑的药动学过程符合二室模型。 This article reports the use of 10% trichloroacetic acid (TCA) both as a plasma protein precipitant and metronidazole extraction solvent, measured at 320nm after precipitation of plasma protein 10% TCA liquid and its lye differential absorbance differential spectroscopy Luminosity method. This method is simple, sensitive and reproducible, which can eliminate the interference of ultraviolet absorbing substances in plasma and meet the requirements of clinical liquid drug monitoring and pharmacokinetic studies. According to the determination of animal plasma concentrations of metronidazole pharmacokinetic process in line with two-compartment model.