论文部分内容阅读
目的研究男男性行为者(MSM)艾滋病(AIDS)病人急性期、慢性期外周血单核细胞(PBMC)中的干扰素(IFN)及其JAK-STAT信号传导通路中相关分子,以期发现IFN-λs、IFN-λR及相关基因、蛋白在HIV不同感染时期的变化规律。方法收集PBMC标本,用实时荧光定量聚合酶链反应(RT-PCR)及流式细胞术方法分析IFN-α/βR2、IFN-γReceptor 1、IFN-λR1、STAT1、STAT2、STAT3、STAT4、STAT5a、STAT5b、STAT6、IRF9、Mx1、OAS1信使核糖核酸(mRNA)及蛋白的表达。结果对20例急性HIV-1感染(AHI)病人、24例慢性HIV-1感染(CHI)病人、22例健康对照(HC)的PBMC标本进行分析,RT-PCR结果显示,在急性HIV-1感染患者中,IFN-αR2、IFN-γReceptor1、IFN-λR1、STAT1、MX1的mRNA水平高于健康对照组(分别为6.03、9.89、8.20、7.03、5.89倍)(P<0.01)。IFN-λR1的mRNA水平与CD4+T淋巴细胞计数呈负相关(R=-0.403,P=0.009),与病毒载量呈正相关(R=0.43,P=0.005)。流式细胞术结果显示,在急性HIV-1感染患者中,IFN-αR2、IFN-γReceptor1、IFN-λR1、STAT1的蛋白水平高于健康对照组(P<0.01)。结论急性HIV-1感染可以提高IFN-λR1及相关基因、蛋白的表达,并且与疾病进展相关。
Objective To investigate the expression of interferon (IFN) and its related JAK-STAT signal transduction pathway in peripheral blood mononuclear cells (PBMCs) of acute and chronic phase of MSM in AIDS patients, λs, IFN-λR and related genes, proteins in different HIV infection period changes. Methods PBMC samples were collected and the expression of IFN-α / βR2, IFN-γR1, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, STAT6, IRF9, Mx1, OAS1 messenger RNA (mRNA) and protein expression. Results Twenty PBMC samples from 20 patients with acute HIV-1 infection (AHI), 24 patients with chronic HIV-1 infection (CHI) and 22 healthy controls (HC) were analyzed. The results of RT- Infected patients, the mRNA levels of IFN-αR2, IFN-γReceptor1, IFN-λR1, STAT1 and MX1 were significantly higher than those of the healthy controls (6.03,9.89,8.20 and 7.03, respectively, 5.89 times) (P <0.01). The mRNA level of IFN-λR1 was negatively correlated with CD4 + T lymphocyte count (R = -0.403, P = 0.009), and positively correlated with viral load (R = 0.43, P = 0.005). The results of flow cytometry showed that the protein levels of IFN-αR2, IFN-γReceptor1, IFN-λR1 and STAT1 were higher in patients with acute HIV-1 infection than those in healthy controls (P <0.01). Conclusions Acute HIV-1 infection can increase the expression of IFN-λR1 and related genes and proteins, and is related to the progress of the disease.