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目的:观察维吾尔族宫颈癌患者放疗前、后血清蛋白质组表达的变化。方法:收集维吾尔族宫颈癌患者60例,单纯放射治疗后由完全缓解的42例中随机抽取7例并留取其放疗前、后的血清样本作为放疗前组及放疗后组,同时由60例健康维吾尔族妇女血清样本中随机抽取7例做为正常组,采用蛋白质二维液相色谱系统(Proteome LabTM PF-2D)分别将放疗前组、放疗后组、正常组血清标本相比较,观察血清蛋白质组表达的变化。结果:放疗前组与正常组血清蛋白质组差异表达图谱鉴别13个位点的峰值差异有统计学意义(P<0.05);放疗前组与放疗后组鉴别出11个蛋白峰值差异有统计学意义(P<0.05);放疗后组与正常组仅2个蛋白峰值差异有统计学意义(P<0.05)。结论:维吾尔族宫颈癌患者经放疗后完全缓解者的蛋白质表达接近正常水平;通过对维吾尔族宫颈癌患者放疗前、后蛋白质组的测定,有可能对治疗效果的判断及早期复发的预测等方面提供有用的信息。
Objective: To observe the changes of serum proteome in Uygur cervical cancer patients before and after radiotherapy. Methods: Sixty Uygur patients with cervical cancer were collected. Seven patients were randomly selected from 42 patients with complete remission after radiotherapy and the serum samples before and after radiotherapy were taken as the pre-radiotherapy group and post-radiotherapy group. Sixty patients Serum samples of healthy Uygur women were randomly selected from 7 patients as normal group. Serum samples of pre-radiotherapy group, radiotherapy group and normal group were compared by protein two-dimensional liquid chromatography (Proteome LabTM PF-2D) Changes in proteome expression. Results: There were significant differences in the peak value of 13 loci between differential expression profiles of serum proteomes before radiotherapy and normal group (P <0.05). There was significant difference in peak value of 11 proteins between radiotherapy group and radiotherapy group (P <0.05). There was only difference between the two groups after radiotherapy (P <0.05). Conclusion: The protein expression of uterine cervix cancer patients with complete remission after radiotherapy is close to the normal level. Through the determination of proteome of Uygur cervical cancer patients before and after radiotherapy, it is possible to judge the therapeutic effect and predict the early recurrence Provide useful information