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研究猪苓多糖对小鼠骨髓树突状细胞(DC)表型及功能成熟的影响。从小鼠骨髓中分离单个核细胞(MNC),用rmGM-CSF、IL-4培养5 d后,实验组加入猪苓多糖(50μg/ml),空白对照组加等量RPMI 1640,阳性对照组加LPS(1μg/ml),3组分别同时作用48 h。扫描电镜观察其体外培养过程中的形态特征,流式细胞仪(FACS)分析其表型特征,混合淋巴细胞反应(MLR)检测其功能。用猪苓多糖刺激的小鼠骨髓MNC具典型的DC形态;细胞表达I-A/I-E、CD11c和分泌IL-12能力比空白对照组增加,具有极强的激发MLR的能力,显示成熟DC的特征。猪苓多糖可以促进体外培养的小鼠骨髓DC的成熟,促进DC诱导的免疫应答启动。
To study the effect of Polyporus umbellatus polysaccharide on the phenotype and functional maturation of mouse bone marrow dendritic cells. Mononuclear cells (MNCs) were isolated from mouse bone marrow. After cultured for 5 days with rmGM-CSF and IL-4, the experimental group received Polyporus umbellatus polysaccharide (50μg / ml), and the blank control group was given the same amount of RPMI 1640. LPS (1μg / ml), 3 groups were treated simultaneously for 48 h. Scanning electron microscopy (SEM) was used to observe the morphological characteristics of the cells cultured in vitro. The phenotypic characteristics were analyzed by flow cytometry (FACS). The mixed lymphocyte reaction (MLR) was used to detect the function of the cells. The mouse bone marrow MNC stimulated with Polyporus umbellatus polysaccharide showed a typical DC morphology. The ability of cells to express I-A / I-E, CD11c and IL-12 was increased compared with the blank control group. Polyporus umbellatus polysaccharide can promote the maturation of mouse bone marrow DC cultured in vitro and promote DC-induced immune response.