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目的 :探讨热休克蛋白 (HSP)对树突状细胞 (DCs)成熟的影响 ,同时对其形态学动态变化进行研究。方法 :从肝癌组织中提取HSP(gp96 )抗原肽复合物 ;肝癌细胞进行热休克处理诱导其表面表达HSP ,再用DiI荧光标记 ;将两种HSP与DCs混合培养 ,动态观察DCs捕获抗原和成熟过程中的形态学变化。流式细胞仪检测不同情况下热休克蛋白对DCs成熟表型的影响。结果 :使用DiI标记的方法良好地显示了DCs摄取抗原的形态学变化 ;DCs通过直接接触、包绕、形成囊泡和伸出伪足且末端形成囊泡 4种方式摄取抗原 ;热休克蛋白可促进DCs成熟表型的表达。结论 :DiI是适合DCs形态学研究的良好的荧光标记物 ;DCs捕获抗原有 4种不同方式 ;不同热休克蛋白均可促进DCs成熟 ,但提取的热休克蛋白作用更显著
Objective: To investigate the effect of heat shock protein (HSP) on the maturation of dendritic cells (DCs), and to study its morphological changes. METHODS: HSP (gp96) antigen peptide complex was extracted from hepatocellular carcinoma tissue. Heat shock treated hepatoma cells were induced to express HSP on the surface and then labeled with DiI. The two HSPs were mixed with DCs to observe the capture antigen and maturation of DCs Morphological changes in the process. Effects of Heat Shock Proteins on Mature Phenotypes of DCs in Different Circumstances by Flow Cytometry. RESULTS: The morphological changes of antigen uptake by DCs were well demonstrated using the DiI-labeled method. DCs uptake the antigen by direct contact, wrapping, vesicle formation and pseudopodia formation and vesicle formation at the end; heat shock proteins promoted DCs mature phenotypes. CONCLUSION: DiI is a good fluorescent marker for morphological studies of DCs. There are four different ways of antigen capture by DCs. Different heat shock proteins can promote the maturation of DCs, but the effect of heat shock protein extraction is more significant