论文部分内容阅读
从临床收集耐亚胺培南的铜绿假单胞菌和鲍曼不动杆菌共50株,进行头孢他啶和2-巯基乙醇的表型协同试验(CAZ+ME),然后进行金属酶IMP-1基因的PCR检测。选取IMP-1阳性株测序,用PCR方法检测有无一类整合子基因(IntI1)。表型的检测发现有28株为协同阳性,其中铜绿假单胞菌27株,鲍曼不动杆菌1株。PCR和测序检测出其中一株铜绿假单胞菌含有IMP-1基因,同时也含有IntI1基因。首次在中国西部地区发现产IMP-1型金属酶、同时也含有一类整合子的铜绿假单胞菌,对于临床上研究细菌的耐药性传播具有重要意义。
A total of 50 strains of Pseudomonas aeruginosa and Acinetobacter baumannii resistant to imipenem were collected clinically for phenotypic synergy test of ceftazidime and 2-mercaptoethanol (CAZ + ME), and then the metalloenzyme IMP-1 gene PCR detection. The IMP-1 positive strains were selected for sequencing and the presence or absence of a class of integron genes (IntI1) was detected by PCR. 28 phenotypes were found to be synergistic, including 27 Pseudomonas aeruginosa and 1 Acinetobacter baumannii. One of the strains, Pseudomonas aeruginosa, contains the IMP-1 gene by PCR and sequencing and also contains the IntI1 gene. For the first time in China’s western region found IMP-1-type metalloenzyme, but also contains a class of integron Pseudomonas aeruginosa, for the clinical study of bacterial resistance spread is of great significance.