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目的:观察lrig1基因在食管鳞癌中的表达及意义.方法:采用RT-PCR检测36例食管鳞状细胞癌癌组织、相应的癌旁组织和远癌组织中lrig1 mRNA的表达情况.PCR产物经凝胶电泳,比较3种组织中lrig1与GAPDH条带的灰度值之比,半定量分析lrig1 mRNA的表达水平.结果:36例食管癌组织中有15例(42%)lrig1 mRNA检测到阳性表达,21例(58%)lrig1 mRNA表达缺失,其阳性表达率低于相应的癌旁组织(92%)和远癌组织(100.0%,P<0.05);癌组织中lrig1 mRNA表达水平(0.76±0.22)低于相应的癌旁组织(0.89±0.33)和远癌组织(1.13±0.40);随着肿瘤分化程度的升高,lrig1 mRNA在癌组织中的阳性表达率也增加(P<0.05),但lrig1 mRNA表达缺失与食管癌临床分期(TNM),淋巴结有无转移和病理类型均无统计学差异(P>0.05).结论:lrig1 mRNA在食管癌组织中存在表达缺失和低表达,提示lrig1基因在食管癌的发生发展中可能具有抑癌基因的作用.
OBJECTIVE: To observe the expression of lrig1 gene in esophageal squamous cell carcinoma and its significance.Methods: The expression of lrig1 mRNA in 36 cases of esophageal squamous cell carcinoma, corresponding paracancerous tissues and distant tissues was detected by RT-PCR.PCR products The expression of lrig1 mRNA was semi-quantitatively analyzed by gel electrophoresis and the ratio of lrig1 to GAPDH bands was compared between the three tissues.Results: Among the 36 cases of esophageal cancer, lrig1 mRNA was detected in 15 cases (42%) The positive expression rate of lrig1 mRNA in 21 cases (58%) was lower than that in corresponding adjacent tissues (92%) and distant cancer tissues (100.0%, P <0.05) 0.76 ± 0.22), which was lower than that in the corresponding paracancerous tissues (0.89 ± 0.33) and distant cancer tissues (1.13 ± 0.40). The positive rate of lrig1 mRNA in cancer tissues increased with the degree of tumor differentiation (P < 0.05), but the expression of lrig1 mRNA was not associated with TNM, lymph node metastasis and pathological type (P> 0.05) .Conclusion: The expression of lrig1 mRNA in esophageal cancer tissues is low and low , Suggesting that lrig1 gene may play a role in tumor suppressor gene in the development of esophageal cancer.