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为明确红树内生解淀粉芽孢杆菌AiL3在大豆体内的定殖及促生效果,通过逐步提高抗生素浓度驯化和平板对峙生长法,筛选对利福平标记稳定且对大豆疫霉菌具有较好抑菌作用的AiL3Rif突变菌株,分别采用灌根和涂叶法研究了AiL3Rif在大豆体内的定殖动态,并在灌根处理中考察了菌株发酵液对大豆的促生作用。结果表明:培养10代后的AiL3Rif标记保持稳定,原始AiL3菌株和AiL3Rif菌株对大豆疫霉抑制率分别为57.58%和56.92%;灌根与涂叶法均可使利福平标记菌株AiL3在大豆体内定殖,且能上下传导,有效定殖期持续达30 d以上,其中灌根处理第11天后大豆根部定殖数量最高可达6.2×103cfu·g-1,同期定殖量根部>茎部>叶片,涂叶处理第1天大豆叶片出现最大值3.34×103cfu·g-1,同期定殖量叶片>茎部>根部,总体看来定殖量灌根要高于涂叶同期处理,且相对稳定;促生方面,以浓度为1.0×107cfu·mL-1AiL3菌株悬浮液灌根盆栽大豆,20 d后处理组鲜重、干重、株高、根长、叶绿素、根系活力等指标均显著高于其他组,说明红树内生细菌AiL3对大豆具有明显促生作用。
In order to clarify the colonization and promoting effect of endophytic Bacillus amyloliquefaciens AiL3 in soybean, through the gradual increase of antibiotic concentration domestication and plate confrontation growth method, the screening of rifampicin was stable and had good inhibitory effect on Phytophthora sojae The strain AiL3Rif was used to study the colonization of AiL3Rif in soybean by using the method of irrigation and leaf application. The promoting effect of the fermentation broth on soybean was also investigated in the treatment of root-filling. The results showed that the AiL3Rif marker was stable after 10 generations of culture. The inhibition rates of the original strain AiL3 and AiL3Rif on Phytophthora sojae were 57.58% and 56.92% In vivo colonization and up and down conduction, the effective colonization period continued for more than 30 d, of which 11 days after rooting treatment soybean root colonization up to a maximum of 6.2 × 103cfu · g-1, the same time the root volume of colonization> stem > Leaves, leaves on the first day of soybean leaf maximum 3.34 × 103cfu · g-1, the same colonization of the amount of leaves> stem> roots, colonies generally appear to rooting than the same period of treatment, and In the aspect of promoting growth, the fresh weight, dry weight, plant height, root length, chlorophyll, root vigor and other indexes were all significantly increased after 20 days of treatment with potted soybean at the concentration of 1.0 × 107cfu · mL-1AiL3. Higher than other groups, indicating mangrove endophytic bacteria AiL3 on soybean has a significant role in promoting growth.