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目的:研究乌司他丁(UTI)在梗阻性黄疸大鼠模型中保护肠粘膜屏障功能的作用。方法:将56只SD大鼠随机分为假手术组(sham)、阻黄组(OJ)、治疗组(UTI),制模成功后治疗组从尾静脉注射UTI 1 mL(1万U·只~(-1)),1次·d~(-1)。UTI3、UTI6和UTI9组分别连续用3d、6d和9d后处死,sham组、OJ组同期尾静脉注射等量生理盐水后处死;观察一般情况、下腔静脉采血测定血浆内毒素(ET)、TNF-α、WBC计数、细菌易位率、肝功能(ALT、SB),及取肝、小肠标本作病理分析。结果:UTI在治疗梗阻性黄疸大鼠时,与OJ比较,UTI组白细胞(WBC)、谷丙转氨酸(ALT)、总胆红素(SB)、内毒素(ET)、肿瘤坏死因子-α(TNF-α)、细菌易位率、腹水、伤口感染、出血、肝淤胆、肝细胞坏死程度、小肠粘膜水肿、糜烂、萎缩、炎性细胞浸润程度等明显降低。结论:UTI在治疗梗阻性黄疸大鼠时对保护肝功能、抑制全身炎症反应、降低肠道细菌易位率、保护肠粘膜屏障有一定的作用。
Objective: To investigate the effect of ulinastatin (UTI) on the intestinal mucosal barrier function in obstructive jaundice rat model. Methods: Fifty-six SD rats were randomly divided into sham group, OJ group and UTI group. After treatment, the rats in treatment group were injected with 1 mL UTI ~ (-1)), once · d ~ (-1). UTI3, UTI6 and UTI9 groups were sacrificed on the 3rd, 6th and 9th day respectively. The sham group and the OJ group were sacrificed by injecting the same volume of saline into the caudal vein at the same time. Blood samples were collected from inferior vena cava to determine plasma endotoxin (ET), TNF -α, WBC count, bacterial translocation rate, liver function (ALT, SB), and pathological analysis of liver and small intestine specimens. Results: Compared with OJ group, UTI group showed significantly higher WBC, ALT, total bilirubin (SB), endotoxin (ET), tumor necrosis factor - α (TNF-α), bacterial translocation rate, ascites, wound infection, hemorrhage, liver cholestasis, hepatocellular necrosis, intestinal mucosal edema, erosion, atrophy and inflammatory cell infiltration were significantly decreased. Conclusion: UTI plays an important role in protecting liver function, inhibiting systemic inflammatory response, reducing intestinal bacterial translocation rate and protecting the intestinal mucosal barrier in the treatment of obstructive jaundice rats.