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目的:研究双氢青蒿素滴眼液对血管化大鼠角膜磷酸化p38(p-p38)以及血管内皮生长因子A(VEGFA)mR NA表达的影响,探讨其抑制角膜新生血管的可能机制。方法:角膜缝线法建立24只大鼠右眼角膜新生血管模型,随机分为双氢青蒿素滴眼液组和生理盐水组各12只。术后第7天,测量角膜新生血管长度,并计算新生血管面积;2组各随机选取6只大鼠并摘取角膜检测角膜p-p38的表达及VEGFA mR NA的表达。结果:双氢青蒿素滴眼液组大鼠角膜新生血管面积显著低于生理盐水组(P<0.01);双氢青蒿素滴眼液组大鼠角膜p-p38表达量低于生理盐水组;双氢青蒿素滴眼液组大鼠角膜VEGFA mR NA表达量显著低于生理盐水组(P<0.05)。结论:20mg/L双氢青蒿素滴眼液可能通过抑制p-p38的表达以及VEGFA mRNA的转录,从而抑制大鼠角膜新生血管生长;p38信号转导通路可能参与调控大鼠角膜新生血管生长。
Objective: To investigate the effect of dihydroartemisinin eye drops on corneal phosphorylated p38 (p-p38) and vascular endothelial growth factor A (VEGFA) mR NA expression in vascularized rats and its possible mechanism of inhibiting corneal neovascularization. Methods: 24 corneal neovascularization models were established by corneal suture method and were randomly divided into two groups: dihydroartemisinin group and saline group. The corneal neovascularization was measured on the 7th day after surgery. The neovascularization area was calculated. In the 2 groups, 6 rats were randomly selected and the cornea was taken out to detect the expression of p-p38 and VEGFA mR NA. Results: The corneal neovascularization area of dihydroartemisinin eyedrops group was significantly lower than that of the saline group (P <0.01). The expression of p-p38 in the cornea of dihydroartemisinin eyedrops group was lower than that of normal saline Group. The expression of VEGFA mR NA in cornea of dihydroartemisinin group was significantly lower than that of saline group (P <0.05). Conclusion: 20mg / L dihydroartemisinin eye drops may inhibit corneal neovascularization by inhibiting the expression of p-p38 and the transcription of VEGFA mRNA. The p38 signal transduction pathway may be involved in the regulation of corneal neovascularization in rats .