论文部分内容阅读
本研究以57个中国大豆祖先品系及育成品种和18个美国大豆祖先品系为DNA样品来源,通过随机引物PCR扩增基因组DNA的多态性,探索利用RAPD标记鉴定相关种质的可能性。研究结果表明,50个10摩尔随机引物共扩增可分辨产物246个,其中82.4％的随机引物可产生多态性产物,所扩增产物的54.4％至少在两个基因型间存在差异。每个PCR扩增产物分别以1和0记录存在与否。扩增产物间的成对比较可产生非相似性矩阵,此矩阵用于构建遗传相似性的树状系谱图。聚类分析结果表明,中国大豆种质与美国大豆种质不同,两国内的南北方种质也不同。这种明显的地理差异为丰富中国大豆品种遗传基础的种质选择提供了理论依据。
In this study, 57 Chinese soybean ancestral lines and bred varieties and 18 American soybean ancestral lines were used as the source of DNA samples. Random amplified polymorphic DNA was amplified by random primers to explore the possibility of using RAPD markers to identify related germplasm. The results showed that a total of 246 differentially expressed products were amplified by 50 10-mer random primers. Among them, 82.4% of the random primers produced polymorphic products, and 54.4% of the amplified products differed at least between the two genotypes. Each PCR amplification product was recorded as 1 and 0, respectively. Pairwise comparisons between amplified products yielded non-similarity matrices that were used to construct a tree-like map of genetic similarity. Cluster analysis showed that Chinese soybean germplasm was different from that of American soybean germplasm, and the germplasm in both North and South China was also different. This obvious geographical difference provides a theoretical basis for enriching the germplasm selection of the genetic basis of Chinese soybean varieties.