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讨论了从瘦柄红石蕊Cladonia macilenta发酵液中分离得到的一种自然界罕见的菲醌化合物biruloquinone的体外抗氧化能力;分别采用DPPH·自由基清除法和ABTS·+自由基清除法对其抗氧化活性进行测定。结果显示,biruloquinone具有较高的抗氧化活性,且在检测浓度范围内对自由基的清除效果呈现剂量依赖关系,在浓度分别为100μg/m L和1.2 mmol/L时对DPPH·和ABTS·+自由基清除能力最大值分别达到90.83%和75.39%,其自由基半数清除浓度(EC50)分别为24.91μg/m L和0.488 mmol/L,在ABTS·+法中,biruloquinone的总抗氧化能力为2.5 TEAC。
The in vitro antioxidant activity of biruloquinone, a rare phenanthrenequinone compound isolated from the fermented liquid of Cladonia macilenta, was studied. The anti-oxidative activity of biruloquinone, a radical scavenger of DPPH and ABTS · + Oxidation activity was measured. The results showed that biruloquinone had high antioxidant activity and showed a dose-dependent effect on the scavenging effect of free radicals at the concentration range of 100 μg / mL and 1.2 mmol / L, respectively. DPPH · and ABTS · + The maximum scavenging capacity of free radicals reached 90.83% and 75.39% respectively, and the half-value free radicals (EC50) were 24.91μg / m L and 0.488 mmol / L respectively. The total antioxidant capacity of biruloquinone was ABTS · + 2.5 TEAC.