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为了探讨大鼠局灶性脑缺血再灌注缺血半暗带β淀粉样前蛋白 (APP)转录水平与缺血时间及梗死体积的相互关系 ,用插线法建立大鼠局灶性脑缺血再灌注模型 ,剥取缺血半暗带皮质组织 ,采用半定量逆转录 聚合酶链式反应 (RT PCR) ,测定永久性缺血 4 8h和不同缺血时间再灌注 4 8h后 ,APPmRNA水平的变化。结果显示 ,梗死体积随再灌注前缺血时间的延长而增大 ,皮质半暗带缺血 30min再灌注 4 8hAPPmRNA表达升高 ;缺血 6 0min和缺血 12 0min再灌注 4 8hAPPmRNA升高明显 ;缺血 180min再灌注 4 8h和永久性缺血 4 8hAPPmRNA达到高峰。提示缺血半暗带APPmRNA的表达随再灌注前缺血时间延长而增加并与梗死体积有一定的相关性 ,早期再灌注可减少其表达。 ((GFDA1) )
To investigate the relationship between amyloid precursor protein (APP) transcription and ischemic time and infarct volume in focal cerebral ischemia / reperfusion ischemia model in rats, focal cerebral ischemia Blood reperfusion model, the cortex of ischemic penumbra was stripped, and the level of APP mRNA was determined by semi-quantitative reverse transcriptase-polymerase chain reaction (RT PCR) 48 h after permanent ischemia and 48 h after ischemia reperfusion The change. The results showed that the volume of infarction increased with the prolongation of ischemic time before reperfusion, and the expression of APAP mRNA increased at 48 min after reperfusion of 30 min in cortical penumbra. The mRNA of APP increased at 60 min of ischemia and 48 h after reperfusion, 180 min ischemia reperfusion 48h and permanent 48hAPP mRNA peak. It suggested that the expression of APPmRNA in ischemic penumbra increased with the prolongation of ischemia time before reperfusion, and had some correlation with infarct volume. Early reperfusion could reduce the expression of APPmRNA. ((GFDA1))