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目的：探讨加用膳食纤维的肠内营养（ＥＮ）对短肠大鼠结肠代偿的促进作用。方法：制作切除８０％－８５％的短肠大鼠模型，用 Ｐｅｐｔｉ－２０００做 ＥＮ治疗，同时加用膳食纤维（ＥＦ），观察其全身营养状况和结肠形态学的改变。结果：术后 １５ ｄ起ＥＦ组体重增加，并于第１８ ｄ起明显优于ＥＮ组（Ｐ＜０ ０５），第９天ＥＮ组正氮平衡明显低于ＣＯＮＴ组（Ｐ＜０ ００１），而ＥＦ组与 ＣＯＮＴ组无差异。 ＥＦ组结肠壁厚度、粘膜厚度、绒毛高度和绒毛表面积均比 ＣＯＮＴ和 ＥＮ组有极为显著的差异（Ｐ＜ ０．０１）。结肠粘膜细胞ＤＮＡ指数，ＥＮ和ＥＦ组均明显高于ＣＯＮＴ组（Ｐ＜０．０５），且Ｓ期细胞百分比均明显高于ＣＯＮＴ组（Ｐ＜０． ０５）。各实验组 ＩＧＦ— １ｍ ＲＮＡ含量均比 ＣＯＮＴ组有极显著差异（ｐ＜ ０．０１）。 ＥＦ组血胰岛素和胰高糖素明显高于 ＣＯＮＴ组（ Ｐ＜ ０． ０５）。结论：应用膳食纤维治疗的短肠大鼠全身营养状况及结肠形态学代偿明显优于单用肠内营养组。
Objective: To investigate the effect of enteral nutrition (EN) supplemented with dietary fiber on colonic compensatory in short-term rats. Methods: 80% -85% of short-cut rat models were made. EN-treated with Pepti-2000 and dietary fiber (EF) were added to observe the changes of their nutritional status and colon morphology. Results: The body weight of EF group increased from the 15th day after operation and was significantly better than that of EN group on the 18th day (P <0 05). On the 9th day, the positive nitrogen balance of EN group was significantly lower than that of the CONT group (P <0.001) There was no difference between EF group and CONT group. The thickness of colon wall, mucosal thickness, villus height and villus surface area in EF group were significantly different from those in CONT and EN group (P <0.01). The DNA index of colonic mucosal cells in EN group and EF group were significantly higher than that in CONT group (P <0.05), and the percentage of cells in S phase was significantly higher than that in CONT group (P <0.05). The levels of IGF-1m RNA in each experimental group were significantly different from those in CONT group (p <0.01). The levels of blood insulin and glucagon in EF group were significantly higher than those in CONT group (P <0.05). Conclusion: The nutritional status of the whole body and the colon morphological compensation of short-term rats treated with dietary fiber are obviously better than those of enteral nutrition group.