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目的探讨当归补血汤超滤物延缓大鼠心脏衰老的作用及其机理,并比较不同分子量范围超滤物的作用效果。方法健康Wistar青年雄性大鼠12只作为青年组,将72只Wistar老年雄性大鼠随机分为6组,每组12只,分别为衰老对照组、当归补血汤超滤膜提取物(0~5万,0~10万,0~20万分子量)组、当归补血汤水煎剂组、维生素E组,给药组连续灌胃10周后,利用酶联免疫吸附法检测各组心肌组织中LPF含量水平;光镜下观察心脏病理改变;电镜下观察心肌超微结构;PCR法检测心肌组织mtDNA缺失。结果与衰老对照组相比,当归补血汤超滤膜提取物0~5万、0~10万、0~20万分子量组、当归补血汤水煎剂、维生素E组的心肌组织中LPF含量水平不同程度降低(P<0.05);心肌组织中mtDNA缺失水平不同程度降低(P<0.05);在组织形态学方面观察到了心肌横纹结构及线粒体形态的差异;各用药组间比较,0~10万分子量组效果最为显著。结论当归补血汤超滤物具有延缓大鼠心脏衰老的作用,其发挥作用的最佳有效组分在0~10万分子量,其通过减少心肌组织中mtDNA缺失,维持细胞内线粒体正常功能发挥抗衰老作用。
Objective To investigate the role and mechanism of Dangguibuxuetang UF in delaying heart aging in rats and compare the effects of ultrafiltration with different molecular weight. Methods Twelve young male Wistar rats were randomly divided into 6 groups (n = 12 in each group): aging control group, ultrafiltration extract of Dangguibuxuetang (0 ~ 5 10000, 0 ~ 200000), and the decoction of Dangguibuxuetang decoction group, vitamin E group and administration group for 10 weeks. The content of LPF in myocardial tissue of each group was detected by enzyme-linked immunosorbent assay The pathological changes of heart were observed under light microscope. The ultrastructure of myocardium was observed under electron microscope. The mtDNA deletion in myocardium was detected by PCR. Results Compared with aging control group, the levels of LPF in myocardial tissue of Dangguibuxuetang ultrafiltration membrane extract from 0 to 50000, from 0 to 100,000, from 0 to 200,000, and from Dangguibuxuetang decoction and vitamin E group were different (P <0.05). The level of mtDNA deletion in myocardium decreased to some extent (P <0.05). The histomorphology of myocardial striated pattern and mitochondria were observed. The most effective molecular weight group. Conclusion Dangguibuxuetang ultrafiltrated can delay the heart aging in rats. The best effective component of Dangguibuxuetang decoction is in the range of 0-100,000. It can play an anti-aging role by reducing the mtDNA loss in myocardium and maintaining the normal function of mitochondria in cells effect.