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为了研究具有临床应用前景的 Ａ Ｓ Ｏ Ｄ Ｎ 脂质体转运系统，以临床药用大豆磷脂为主要原料制备了ｐ Ｈ 敏脂质体，并测定了脂质体体外转染活性、ｐ Ｈ 敏特性、细胞毒性和对 Ａ Ｓ Ｏ Ｄ Ｎ 抗流感病毒活性的影响 结果发现，批号为 ９８０５１９０３，９８０５１１０２ 和 ９８０５１２０２ 的脂质体具有较高转染活性，但只有ｌｉｐｏｆｅｃｔｉｎ 转染活性的 １／５０～１／１００当质粒／脂质体（ Ｗ ／ Ｗ ）为 １∶４～１∶８，转染时间为 ３～５ ｈ，质粒量为 ０５ μｇ，转染后 ２４～４８ ｈ 内检测时转染活性最高 脂质体 ９８０５１２０２ 表现明显 ｐ Ｈ值依赖溶解红细胞膜特性，而脂质体 ９８０５１１０２ 和 ９８０５１９０３ 的 ｐ Ｈ 敏特性不明显 脂质体细胞毒性明显降低，如 ９８０５１９０３、９８０５１１０２ 和 ９８０５１２０２ 的毒性分别是 ｌｉｐｏｆｅｃｔｉｎ 毒性的 １／１６、１／８ 和 １／４ｐ Ｈ 敏脂质体 ９８０５１２０２ 具有促进 Ａ Ｓ Ｏ Ｄ Ｎ 抗流感病毒作用，当 Ａ Ｓ Ｏ Ｄ Ｎ 浓度为 ０２ μｍ ｏｌ／ Ｌ 时，ｐ Ｈ 敏脂质体 ９８０５１２０２ 使其抗病毒活性提高 ５ 倍，但 Ａ Ｓ Ｏ Ｄ Ｎ 浓度较高时ｐ Ｈ 敏脂质体对 Ａ Ｓ Ｏ Ｄ Ｎ抗?
In order to study A S O D N liposome transport system with clinical application prospect, p H-sensitive liposomes were prepared from clinical medicinal soybean lecithin as the main raw material, and the in vitro transfection activity of liposomes was determined. The results showed that the liposomes with lots 98051903, 98051102 and 98051202 had higher transfection activity, but only those with lipofectin transfection activity of 1/50 ~ 1/100. When the plasmid / liposome (W / W) was 1: 4 to 1: 8, the transfection time was 3 to 5 h, the amount of plasmid was 0 5 μg, and it was detected within 24-48 h after transfection Liposome 98051202 showed the highest p H value depending on the characteristics of lysed erythrocyte membrane, whereas the p H sensitivities of liposomes 98051102 and 98051903 were not obvious The cytotoxicity of liposomes was significantly reduced, such as 98051903,980511 02 and 98051202 were lipofectin-toxic 1 / 16,1 / 8 and 1/4 hp liposomes 98051202 has the role of promoting anti-influenza A S O D N virus, when the concentration of A S O D N is 0 2 μm ol / L, p H-sensitive liposome 98051202 made its antiviral activity increased by 5 times, but the high concentration of A S O D N p H-sensitive liposomes against A S O D N?