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量子点一元激发、多元发射的特性能有效地应用到生化多通量检测领域.首先制备高荧光效率的多色核/壳量子点,制备出的油容性量子点经双亲性高分子自组装水溶性改性后连接两种不同的IgG抗体分子,并对相应的IgG抗原以及抗原混合样品同时进行检测.荧光共振能量转移(FRET)是多色量子点在多通量检测中应极力避免的.光谱分析证实,所制备的多色量子点在多通量检测中不会发生荧光共振能量转移.免疫荧光体外检测结果表明,多色量子点-抗体复合物对抗原样品具有特异的检测能力,且非特异性吸附甚少.本模型的建立为多色量子点在生化多通量检测方面提供了科学依据.
Quantum dot one-dimensional excitation, multiple emission characteristics can be effectively applied to the field of biochemical detection of multi-flux.First, a high fluorescence efficiency of multicolor nuclear / shell quantum dots, the prepared oil-containing quantum dots by amphiphilic self-assembly Two different IgG antibody molecules are linked after water-soluble modification, and the corresponding IgG antigen and antigen mixed sample are simultaneously detected. Fluorescence resonance energy transfer (FRET) is a multi-color quantum dot that should be avoided in multi-flux detection The results of spectroscopic analysis confirmed that no fluorescence resonance energy transfer occurred in the multi-flux detection of the multicolor QDs prepared in vitro.The results of immunofluorescence in vitro showed that the multi-color quantum dot-antibody complex has specific detection ability on the antigen sample, And the non-specific adsorption is very little.The establishment of this model provides a scientific basis for biochemical multi-flux detection of multicolor quantum dots.