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电镜酶细胞化学是正在发展中的技术,至今只有近30年的历史。由于酶细胞化学的技术比较复杂,影响因素较多,方法也不够稳定,所以迄今在全国电镜室均未普遍开展。近几年来随着科学技术的发展,特别在生物医学中人们正注意把传统的形态描述与功能相结合起来的要求越来越引起人们的关注,并且有一些方法已开始在临床诊断和研究中使用。作者自1984年则开始探索超微结构细胞化学实验工作,并付之应用。如我们对AKP(碱性磷酸酶)、5′-Nase(5′-核苷酸酶)、MPO(髓过氧化物酶)、ATPase
Electron microscopy Enzyme cytochemistry is a developing technology that has only been in existence for nearly 30 years. As the technology of enzyme cell chemistry is more complicated, more influencing factors, the method is not stable enough, so far in the national electron microscope room are not generally carried out. In recent years, with the development of science and technology, people are paying more attention to the combination of traditional morphological description and function, especially in biomedicine, and some methods have begun to be used in clinical diagnosis and research use. Since 1984, the author has started to explore the experimental work of ultrastructural cytochemistry and pay for it. As we analyzed AKP (alkaline phosphatase), 5’-Nase (5’-nucleotidase), MPO (myeloperoxidase), ATPase