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目的建立大鼠血浆中甘草苷、花椒毒酚和甲基麦冬黄烷酮A的UPLC-MS/MS测定方法,并探讨大鼠灌胃沙参麦冬汤后其在大鼠体内的药动学过程。方法以流动相乙腈-0.1%甲酸水溶液,梯度洗脱,流速0.3 m L·min?1;采用ESI源,正负离子同时检测模式扫描,多反应监测模式(MRM)检测各成分血药浓度,并用DAS 3.0软件计算药动学参数。结果甘草苷、花椒毒酚和麦冬黄烷酮A分别在4.92~315.00 ng·m L?1、1.44~92.00 ng·m L?1和0.35~22.00 ng·m L?1线性关系良好,平均回收率均>76.5%,日内、日间RSD均<15%。大鼠灌胃沙参麦冬汤提取物后,甘草苷、花椒毒酚和甲基麦冬黄烷酮A的AUC0-t分别为(718.23±185.55)ng·h·m L?1,(22.52±7.53)ng·h·m L?1和(13.55±6.03)ng·h·m L?1;t1/2分别为(3.61±2.01)h,(6.93±7.78)h和(3.51±1.92)h。结论本法方便、快捷,可用于甘草苷、花椒毒素和甲基麦冬黄烷酮A的体内定量分析。
OBJECTIVE To establish a method for the determination of liquiritin, prickly ash and methyl isofenone in rat plasma by UPLC-MS / MS and investigate the pharmacokinetics Learn process. Methods The mobile phase of acetonitrile-0.1% formic acid was used as a gradient elution at a flow rate of 0.3 m L · min -1. The ESI source and positive and negative ion simultaneous detection mode were used to detect the plasma concentration of each component. Multiple reaction monitoring (MRM) DAS 3.0 software calculates pharmacokinetic parameters. Results The results showed that the linear relationships of glycyrrhizin, zeaxanthin and Ophiopogon flavonoids A were 4.92 ~ 315.00 ng · m L -1, 1.444 ~ 92.00 ng · m L -1 and 0.35 ~ 22.00 ng · m L -1, respectively. The average Recovery rates were> 76.5%, day and day RSD were <15%. The AUC0-t of glycyrrhizin, prickly ash toxin and methylorthosorbide A were (718.23 ± 185.55) ng · h · m L · 1, (22.52 (3.61 ± 2.01) h, (6.93 ± 7.78) h and (3.51 ± 1.92) ± (3.13 ± 2.81) h and (13.55 ± 6.03) ng · h · m L · 1, h Conclusion This method is convenient and rapid and can be used for in vivo quantitative analysis of liquiritin, zeatoxin and methyl Ophiopogon flavone A.