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应用流式细胞术和细胞免疫荧光染色技术,对胆囊癌前病变和胆囊癌细胞rasP21的表达量进行了定量研究,并分析P21~(ras)的表达量与DNA倍体和细胞增殖指数的关系。结果表明,胆囊癌细胞P21~(ras)表达量(荧光指数FI)明显高于胆囊癌前病变的表达量,并发现P21~(ras)表达的荧光指数随组织学分级增高而升高。DNA倍体与P21ras表达量有密切关系
Flow cytometry and cellular immunofluorescence staining techniques were used to quantitatively study the expression of premalignant gallbladder lesions and rasP21 in gallbladder cancer cells, and the relationship between the expression of P21 ras and DNA ploidy and cell proliferation index was analyzed. . The results showed that the expression of P21 ras (fluorescence index FI) in gallbladder carcinoma cells was significantly higher than that in gallbladder precancerous lesions, and the fluorescence index of P21 ras expression was increased with histological grading. DNA ploidy is closely related to the expression of P21ras