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基于硼砂缓冲液中聚丙烯酰胺(PAM)存在下,邻苯三酚红(PGR)于滤纸固体基质上能发射强而稳定的室温燐光,PAM可活化钴离子催化H2O2氧化PGR反应,使PGR的室温燐光猝灭,从而建立了测定痕量钴的新方法。该方法的线性范围为4.8~960(×10-18g/斑)Co2+(浓度范围0.012~2.40×10-9g/L,0.4μL/斑),工作曲线的回归方程ΔIp=12.76+0.1248 mCo2+(×10-18g/斑),n=7,相关系数r=0.9993;检出限为1.2×10-18g/斑(对应浓度为3.0×10-12g/L)。对4.8和960(×10-18g/斑)Co2+重复测定11次(0.4μL点样量),RSD分别为3.6%和4.7%。用PAM比不加PAM时ΔIp增加4.6倍。本方法灵敏、准确、简便快速、重现性好,用于党参、当归、川芎、金银花和VB12中的痕量钴测定,结果满意。
Based on the presence of polyacrylamide (PAM) in borax buffer, pyrogallol red (PGR) can emit strong and stable catalase on a solid substrate of filter paper. PAM activates cobalt ions to catalyze H2O2 to oxidize PGR and make PGR Room temperature 燐 light quenching, thus establishing a new method for the determination of trace amounts of cobalt. The linear range of this method is 4.8 ~ 960 (× 10-18g / spot) Co2 + (concentration range 0.012 ~ 2.40 × 10-9g / L, 0.4μL / spot). The regression equation of the working curve is ΔIp = 12.76 + 0.1248 mCo2 + 10-18g / spot), n = 7, the correlation coefficient r = 0.9993; the detection limit was 1.2 × 10-18g / spot (the corresponding concentration was 3.0 × 10-12g / L). Eleven measurements (0.4 μL spotting) of Co2 + at 4.8 and 960 (× 10-18 g / spot) were performed with RSDs of 3.6% and 4.7%, respectively. ΔIp increased 4.6 times with PAM than without PAM. The method is sensitive, accurate, simple and rapid, reproducible, and is used for the determination of trace cobalt in Codonopsis pilosula, Angelica sinensis, Chuanxiong rhizome, honeysuckle and VB12 with satisfactory results.