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采用高效液相色谱法(HPLC)可以准确测定淀粉酶法合成海藻糖反应中的海藻糖产率,色谱柱REZEXRNMCARBOHYDRATE,水为流动相,流速0.6ml/min,柱温70℃。试验结果证明,此工艺转化淀粉生产海藻糖产率可达82.07%。
High-performance liquid chromatography (HPLC) can accurately determine the yield of trehalose in the enzymatic synthesis of trehalose by amylase. Column REZEXRNMCARBOHYDRATE, water as the mobile phase, flow rate 0.6ml / min, column temperature 70 ℃. Test results show that the conversion of starch production process trehalose up to 82.07%.