目的:建立高效液相色谱法测定复方罗布麻片Ⅰ中维生素B_6含量的方法。方法:采用Agilent HC-C_(18)色谱柱,乙腈-庚烷磺酸钠溶液[取庚烷磺酸钠2.87 g与磷酸二氢钾2.5 g加水溶解稀释至1000 ml,用磷酸(1→3)调节pH至3.5](15∶85)为流动相,柱温:16℃,检测波长为290 nm,流速1.0 ml·min~(-1)。结果:维生素B_6在3.9～118.3μg·ml~(-1)范围内与峰面积呈良好的线性关系(r=0.999 9),平均回收率为99.1%,RSD=0.80%(n=6)。结论:本法简便、准确、重复性好,可用于该制剂的质量控制。 Objective: To establish a method for the determination of vitamin B 6 in compound Apocynacaptilum I by high performance liquid chromatography. Methods: Agilent HC-C_ (18) column, acetonitrile-heptane sulfonate solution [2.78 g of heptane sulfonate and 2.5 g of potassium dihydrogen phosphate were dissolved in water and diluted to 1000 ml with phosphoric acid (1 → 3 ) To adjust the pH to 3.5] (15:85) as the mobile phase. The column temperature was 16 ℃, the detection wavelength was 290 nm and the flow rate was 1.0 ml · min -1. RESULTS: Vitamin B 6 showed a good linear relationship with the peak area in the range of 3.9-118.3 μg · ml -1 (r = 0.999 9) with an average recovery of 99.1% and a RSD of 0.80% (n = 6). Conclusion: This method is simple, accurate, reproducible, and can be used for the quality control of the preparation.