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[目的]研究转染小凹蛋白-1(Caveolin-1,CAV-1)基因对脂多糖(LPS)诱导的人支气管上皮(HBE)细胞Toll样受体4(TLR-4)表达的影响,以及大黄素对CAV-1和TLR-4表达的干预效应。[方法]本实验分为2部分:第1部分,对LPS诱导的HBE细胞给予不同剂量的大黄素干预,并设立正常对照、阳性对照(阿托伐他汀)和阴性对照(溶媒DMSO),用Western blot检测各组细胞CAV-1的表达水平;第2部分,通过构建CAV1-EGFP重组质粒,瞬时转染HBE细胞,于转染48h后给予LPS刺激,并给予大黄素干预转染后LPS诱导的HBE,Western blot检测各组细胞TLR-4的表达。[结果]中、高剂量的大黄素均能抑制LPS诱导的HBE细胞CAV-1的表达;与各对照组相比,CAV-1过表达能上调LPS诱导的HBE细胞TLR-4的表达,大黄素能抑制该效应。[结论]转染CAV-1基因能上调LPS诱导的HBE细胞TLR-4的表达,大黄素能抑制LPS诱导的HBE细胞CAV-1与TLR-4的表达。
[Objective] To investigate the effect of caveolin-1 (CAV-1) gene on TLR-4 expression induced by lipopolysaccharide (LPS) in human bronchial epithelial cells (HBE) And the effect of emodin on the expression of CAV-1 and TLR-4. [Method] The experiment was divided into two parts: In part 1, different doses of emodin were administered to LPS-induced HBE cells, and normal control, positive control (atorvastatin) and negative control (DMSO) Western blot was used to detect the expression level of CAV-1 in each group. In the second part, the recombinant plasmid of CAV1-EGFP was transiently transfected into HBE cells and stimulated with LPS 48h after transfection. Emodin was used to induce LPS-induced transfection HBE was used to detect the expression of TLR-4 in each group by Western blot. [Results] Emodin at both medium and high doses could inhibit the expression of CAV-1 in LPS-induced HBE cells. Compared with each control group, CAV-1 overexpression up-regulated the LPS-induced TLR-4 expression in HBE cells. Vegetarian can inhibit this effect. [Conclusion] Transfection of CAV-1 gene can up-regulate the expression of TLR-4 induced by LPS in HBE cells. Emodin can inhibit the expression of CAV-1 and TLR-4 induced by LPS in HBE cells.