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目的探讨洛铂对人胃癌细胞株SGC7901放射敏感性的影响。方法 MTT法获得洛铂对人胃癌细胞株SGC7901的半数抑制浓度(IC50),分A组(洛铂4.0μg/ml+0、2、4、6、8Gy照射)和B组(0、2、4、6、8Gy照射),计算洛铂对细胞的放射增敏比(SER)。人胃癌细胞株SGC7901分为洛铂4.0μg/ml+4Gy照射(C组)、4Gy照射(D组)和空白对照(E组),流式细胞术检测三组细胞凋亡率,Western blot测定三组细胞凋亡相关蛋白B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)和活化型半胱天冬酶-3(Caspase-3)表达水平。结果洛铂的IC50为20.66μg/ml,洛铂的SER为1.08。C、D组细胞凋亡率高于E组(P<0.01),C组凋亡率高于D组(P<0.05)。C、D组Bcl-2表达低于E组,而Bax、活化型Caspase-3表达高于E组(P<0.01)。C组Bcl-2表达低于D组,而Bax、活化型Caspase-3表达高于D组(P<0.05)。结论洛铂对人胃癌细胞株SGC7901具有放射增敏作用。其作用机制可能是抑制SGC7901细胞Bcl-2表达,激活Bax表达,与线粒体通路Bcl-2/BaxCaspase信号传导有关。
Objective To investigate the effect of lobaplatin on the radiosensitivity of human gastric cancer cell line SGC7901. Methods MTT assay was used to determine the IC50 values of Losoplatin in human gastric cancer cell line SGC7901. The cells were divided into group A (4.0μg / ml for lobaplatin and 0,2,4,6,8Gy for irradiation) and group B (0,2, 4, 6, 8 Gy irradiation), the radiosensitization ratio (SER) of lobaplatin to cells was calculated. The human gastric cancer cell line SGC7901 was divided into three groups: Losartan 4.0μg / ml + 4Gy irradiation (group C), 4Gy irradiation (group D) and blank control group (E). Flow cytometry was used to detect the apoptosis rate of three groups. Western blot The expressions of Bcl-2, Bcl-2 and Caspase-3 in the three groups were compared. Results The IC50 of lobaplatin was 20.66μg / ml, and the SER of lobaplatin was 1.08. The apoptosis rate of C and D group was higher than that of E group (P <0.01), and the apoptosis rate of C group was higher than that of D group (P <0.05). The expression of Bcl-2 in group C and D was lower than that in group E, while the expression of Bax and activated Caspase-3 was higher in group C than group E (P <0.01). The expression of Bcl-2 in group C was lower than that in group D, while the expression of Bax and activated Caspase-3 was higher in group C than in group D (P <0.05). Conclusion Losplatin has a radiosensitizing effect on human gastric cancer cell line SGC7901. Its mechanism may be inhibition of Bcl-2 expression in SGC7901 cells, activation of Bax expression, and the mitochondrial pathway Bcl-2 / BaxCaspase signaling.