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SNPs能改变基因表达,导致编码氨基酸突变,影响蛋白质功能,这是目前被密切关注的问题.对此,普遍认为蛋白质结构的变化源自氨基酸取代,很少有人注意mRNA结构变化的影响.选取人类??珠蛋白基因HBB及其4个突变体样本,用动态延伸折叠方法构建mRNA二级结构,从有关数据库获得它们的表达产物的二级结构.分别对成熟mRNA和蛋白质做突变前后的结构对比,并进行mRNA-蛋白质相应结构变化的对照.结果发现,蛋白质中规则构象一般为mRNA中的稳定区域编码,而不规则构象(?/?转角、卷曲)为mRNA中的不稳定区域编码.不稳定区域内的碱基突变引起mRNA二级结构的变化,将会在蛋白质结构中留下“变化印迹”.这种mRNA二级结构和编码蛋白质二级结构的相关性可能作为探寻蛋白质功能变化的一种谋略.
SNPs can change the gene expression, leading to mutations in amino acid encoding, affecting protein function, which is currently a matter of close attention.To this, it is generally believed that changes in protein structure derived from amino acid substitution, few people pay attention to changes in mRNA structure.Selecting human The globin gene HBB and its 4 mutants were used to construct the secondary structure of mRNA by the dynamic extension and folding method and to obtain the secondary structure of their expressed products from the relevant database.The structure comparison of the mature mRNA and protein before and after mutation , And a comparison of the corresponding structural changes in mRNA-protein results showed that regular conformations in the protein are generally coding for a stable region in mRNA while irregular conformation (? /?, Curl) is coding for an unstable region in the mRNA. Mutations in the stable region cause changes in the secondary structure of the mRNA, which will leave a “blot” on the protein structure. "The correlation between this mRNA secondary structure and the encoded protein secondary structure may serve as a proxy for protein function A strategy of change.