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目的探讨三氧化二砷（Ａｓ２Ｏ３）对急性早幼粒细胞性白血病（ＡＰＬ）细胞组织因子（ＴＦ）表达的影响。方法利用复钙时间检测使用１μＭＡｓ２Ｏ３处理ＡＰＬ细胞株ＮＢ４细胞以及使用Ａｓ２Ｏ３治疗中不同时间的ＡＰＬ病人的骨髓细胞的促凝活性（ＰＣＡ），并用ＥＬＩＳＡ方法检测其ＴＦ抗原、利用半定量ＲＴＰＣＲ检测ＴＦｍＲＮＡ的转录水平。结果Ａｓ２Ｏ３可以以时间依赖的方式下调ＡＰＬ细胞的促凝活性、ＴＦ抗原水平以及ＴＦｍＲＮＡ的转录；此外，通过对ＰＣＲ产物进行测序，发现ＡＰＬ细胞中还存在ＴＦ第五个外显子缺失的转录本，其生物学意义尚不明了。结论Ａｓ２Ｏ３可下调ＡＰＬ细胞ＴＦ的表达；而且在使用Ａｓ２Ｏ３诱导ＡＰＬ细胞凋亡的同时，Ａｓ２Ｏ３有可能通过下调ＡＰＬ细胞ＴＦ的表达而预防ＤＩＣ的发生
Objective To investigate the effect of arsenic trioxide (As2O3) on the expression of tissue factor (TF) in acute promyelocytic leukemia (APL) cells. Methods The time of recalcification was used to detect the procoagulant activity (PCA) of bone marrow cells from APL patients treated with 1μM S2O3 and APL patients treated with As2O3. The TF antigen was detected by ELISA and detected by semi-quantitative RT PCR. The transcription level of TF mRNA. Results As2O3 could downregulate the procoagulant activity of APL cells, TF antigen level, and transcription of TF mRNA in a time-dependent manner. In addition, by sequencing the PCR products, it was found that transcripts of the fifth exon deletion of TF existed in APL cells. The biological significance is still unknown. Conclusion As2O3 can down-regulate the expression of TF in APL cells, and As2O3 may prevent DIC by down-regulating the expression of TF in APL cells while As2O3 is used to induce apoptosis of APL cells.