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人们已经使用聚合酶链反应来扩增编码不同变异性表面蛋白的基因片断。9个瑞士蓝氏贾第虫无菌虫株被用于基因分析。根据产量、大小和限制性片断长度的多态性分析鉴定3个基因型。其中有5个蓝氏贾第虫株(01,B1,B2,B3-1A1和C1)被分类属于基因组Ⅰ。一个从人类获得的虫株(H2-17A)被分类属于基因组Ⅱ,它产生了一个特殊的VSP1267的PCR产物。这个虫株也产生了1.8kbtsp11和0.52kb类似tsa417/tsp11的PCR产物,这个产物具有基因组Ⅱ型虫株的限制性片断长度的多态性。3个虫株(O2-4A1,O3和H3-15K2)代表了一个新的基因型,它和基因组Ⅰ和基因组Ⅱ密切相关。这3个虫株显示了在tsp11PCR产物中完全相同的限制性片断长度的多态性,同时未能产生1个VSP1267PCR产物。这个结果表明能用这种方法划分肠道蓝氏贾第虫的不同基因型。
Polymerase chain reaction has been used to amplify gene fragments encoding different variant surface proteins. Nine G. gigas strains were used for genetic analysis. Three genotypes were identified based on polymorphism analysis of yield, size and restriction fragment length. Five of the Giardia lamblia strains (01, B1, B2, B3-1A1 and C1) were classified as belonging to genomic I. A human-derived strain (H2-17A) was classified as belonging to Genome II, which produced a special PCR product of VSP1267. This strain also produced a 1.8kbtsp11 and a 0.52kb tsa417 / tsp11-like PCR product with a restriction fragment length polymorphism of the genomic type II strain. Three insect strains (O2-4A1, O3 and H3-15K2) represent a new genotype that is closely related to both Genome I and Genome II. The three insect strains showed the exact same restriction fragment length polymorphism in the tsp11 PCR product while failing to produce one VSP1267 PCR product. This result indicates that this method can be used to separate the different genotypes of Giardia lamblia.