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目的 :比较及分析 3种治疗温病的常用中医治法对家兔内毒素血症的影响。方法 :采用大肠杆菌内毒素 E.Coli O111∶ B4静注复制家兔内毒素血症模型 ,检测血中白介素 1(IL 1)、肿瘤坏死因子 α(TNFα)、前列环素 (PGI2 )、内皮素 1(ET 1)及血浆内毒素 (ETX)等炎性细胞因子及血管活性介质的水平 ,观察清热解毒、活血化瘀、养阴增液 3种温病治法制剂对内毒素血症的治疗效果。结果 :静注内毒素 0 .5小时后模型组动物体温急剧上升 ,IL 1、TNFα、ET 1、ETX水平均明显升高 ,PGI2 下降 ,与正常组比较均有显著性差异(P均 <0 .0 5 )。与模型组比较 ,3个治疗组给予药物治疗后发热等症状得到明显的改善 ;清热解毒组和养阴增液组血浆 IL 1、TNFα、ET 1水平均明显降低 (P均 <0 .0 1) ,而 PGI2 显著升高 (P均 <0 .0 5 ) ,活血化瘀组IL 1水平下降不明显 ,TNFα水平略有上升 ,但其 ET 1、PGI2 改变均非常显著 (P均 <0 .0 1) ;3个治疗组ETX水平无显著变化。结论 :3种温病治法制剂对内毒素血症均有明显的改善作用 ,但其作用途径可能不一致 ,分别通过拮抗内毒素所诱生炎性细胞因子及血管活性介质而阻止内毒素血症的进展。
Objectives: To compare and analyze the effects of three common TCM treatments for treating febrile diseases on endotoxemia in rabbits. METHODS: Endotoxemia model E.coli O111:B4 was used to replicate rabbit endotoxemia with E. coli endotoxemia. Blood IL-1, TNFα, PGI2, and endothelium were detected. The levels of inflammatory cytokines and vasoactive mediators such as ET 1 and plasma endotoxin (ETX) were observed. To observe the effects of heat-clearing, detoxifying, promoting blood circulation, and replenishing yin plus liquid for the treatment of endotoxemia. treatment effect. RESULTS: After 0.5 hours of intravenous injection of endotoxin, the animal body temperature in the model group increased sharply, the levels of IL 1, TNFα, ET 1, and ETX increased significantly, and the PGI 2 decreased. There was a significant difference compared with the normal group (P <0; .0 5 ). Compared with the model group, fever and other symptoms were significantly improved in the three treatment groups after drug treatment; plasma levels of IL 1, TNFα, and ET 1 in the Qingrejiedu group and Yangyinjiang group were significantly lower (P<0.01). PGI2 was significantly elevated (P<0.05). The level of IL-1 in the blood circulation group was not significantly decreased, and the level of TNFα was slightly increased, but the changes of ET1 and PGI2 were all significant (P<0.01. 0 1) There was no significant change in ETX levels in the 3 treatment groups. Conclusion: The three kinds of therapeutic methods for treating diseases with mild diseases have significant effects on improving endotoxemia, but their effects may be inconsistent. They prevent endotoxemia by antagonizing endotoxin-induced inflammatory cytokines and vasoactive mediators, respectively. Progress.