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目的了解产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和克雷伯菌的主要基因型分布特点。方法用表型确证试验筛选出临床标本中产ESBLs的大肠埃希菌和克雷伯菌114株。应用PCR方法扩增产酶株的TEM、SHV和CTX-M基因。结果PCR结果显示TEM、SHV和CTX-M基因的总阳性率分别为40.4%、20.7%和83.3%，其中大肠埃希菌分别为48.1%、1.3%和93.5%，肺炎克雷伯菌分别为24.3%、81.1%和62.2%。46.8%的大肠埃希菌和51.4%的肺炎克雷伯菌同时携带多个基因。结论产ESBLs大肠埃希菌的主要基因型为CTX-M，肺炎克雷伯菌主要基因型为SHV。大多数菌株同时携带多个基因。“,”Objective To investigate the genotypic character of clinical isolates producing extended spectrum beta-lactamases(ESBLs). Methods ESBLs producing Escherichia coli and Klebsiella pneumoniae isolates were indentified by phenotyic confirmatory test. PCR were using to detect TEM, SHV and CTX-M genes. Results The positive rates of TEM, SHV and CTX-M genes of these isolates were 40.4%, 20.7% and 83.3% respectively. Those of Escherichia coli were 48.1%, 1.3% and 93.5% respectively. Those of Klebsiella pneumoniae were 24.3%, 81.1% and 62.2% respectively. 46.8% of Escherichia coli and 51.4% of Klebsiella pneumoniae had more than one genotype. Conclusions The major genotype of ESBLs-producing Escherichia coli was CTX-M and which of Klebsiella pneumoniae was SHV. Many isolates had more than one genotype.