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目的:观察人卵巢癌顺铂(cisplatin,CDDP)敏感细胞株OVCAR-3和耐药细胞株OVCAR-3/CDDP生物学行为的差异,并探讨其可能的机制。方法:取对数生长期的OVCAR-3和OVCAR-3/CDDP细胞,软琼脂克隆形成实验检测细胞的增殖能力;Transwell小室、失巢凋亡实验和裸鼠皮下移植瘤实验分别检测细胞体外和体内的侵袭、迁移能力;免疫组织化学实验检测移植瘤组织中MMP-2和MMP-9的表达。结果:与OVCAR-3细胞比较,OVCAR-3/CDDP细胞克隆形成能力显著增加[(0.66±0.09)%vs(0.31±0.07)%,P<0.05]。Transwell小室实验发现,OVCAR-3/CDDP细胞较OVCAR-3细胞侵袭和迁移能力均明显增强[(233.1±8.5)vs(167.4±5.9),P<0.01;(143.6±9.1)vs(95.8±6.2),P<0.01];OVCAR-3/CDDP细胞较OVCAR-3细胞更易聚集,细胞凋亡指数下降[(7.78±1.32)%vs(15.41±1.26)%,P<0.01]。OVCAR-3/CDDP细胞移植瘤组织中MMP-2、MMP-9的表达高于OVCAR-3细胞。结论:顺铂耐药OVCAR-3/CDDP细胞的增殖、抗失巢凋亡、侵袭和迁移能力显著增强,其机制可能与MMP-2和MMP-9表达升高有关。
OBJECTIVE: To observe the difference of biological behaviors between ovarian cancer cell line OVCAR-3 and OVCAR-3 / CDDP, and to explore its possible mechanism. Methods: The proliferation ability of OVCAR-3 and OVCAR-3 / CDDP cells in logarithmic growth phase was detected by soft agar colony formation assay. Transwell chamber, anoikis test and subcutaneous xenograft tumor in nude mice were used to detect the cell proliferation in vitro and in vitro In vivo invasion and migration; immunohistochemistry was used to detect the expression of MMP-2 and MMP-9 in the xenograft tumor tissue. Results: Compared with OVCAR-3 cells, the colony-forming ability of OVCAR-3 / CDDP cells was significantly increased [(0.66 ± 0.09)% vs (0.31 ± 0.07)%, P <0.05]. Transwell chamber assay showed that the invasion and migration of OVCAR-3 / CDDP cells were significantly enhanced compared with that of OVCAR-3 cells [(233.1 ± 8.5) vs (167.4 ± 5.9), P <0.01; (143.6 ± 9.1 vs 95.8 ± 6.2 ), P <0.01]. OVCAR-3 / CDDP cells were more easily aggregated than OVCAR-3 cells and the apoptotic index decreased ([7.78 ± 1.32]% vs (15.41 ± 1.26)%, P <0.01; The expression of MMP-2 and MMP-9 in OVCAR-3 / CDDP cells was higher than that in OVCAR-3 cells. CONCLUSION: The proliferation, anti-anoikis, invasion and migration of cisplatin-resistant OVCAR-3 / CDDP cells are significantly enhanced, which may be related to the increased expression of MMP-2 and MMP-9.