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目的：以原发性鼻咽癌（NPC）患者为研究对象，检测鼻咽癌组织中P16／MTSI抑癌基因失活情况；研究鼻咽癌发生发展规律。方法：采用PCR、多重PCR、限制性内切酶解一PCR方法对34例鼻咽癌患者和34例非肿瘤（腺样体增殖及慢性炎症）患者，进行P基因纯会缺失和异常甲基化检测。结果：34例NPC患者纯缺失率为8．82％（3／34），异常甲基化率为38．24％（13／34），34例非肿瘤患者纯缺失未检出（0／34），异常甲基化率为8．82％（3／34）。结论：本研究鼻咽癌患者P16／MTSI抑癌基因总失活率为47．06％（16／34），表明P16基因失活在鼻咽癌患者中是较为常见的基因变化。鼻咽癌患者中甲基化异常是P16基因失活的另一重要途径，值得引起重视。
OBJECTIVE: To detect the inactivation of P16 / MTSI tumor suppressor gene in nasopharyngeal carcinoma (NPC) patients with primary nasopharyngeal carcinoma (NPC) and to study the occurrence and development of NPC. Methods: 34 patients with nasopharyngeal carcinoma and 34 patients with non-neoplastic (adenoid proliferation and chronic inflammation) were subjected to PCR, multiplex PCR and restriction endonuclease-PCR for deletion of P gene and abnormal methyl Detection. Results: The pure deletion rate of 34 NPC patients was 8.82% (3/34), the abnormal methylation rate was 38.24% (13/34), and the pure deletion of 34 non-tumor patients was not detected (0/34 ), Abnormal methylation rate was 8.82% (3/34). Conclusion: In this study, the total inactivation rate of P16 / MTSI tumor suppressor gene was 47.06% (16/34), indicating that the inactivation of P16 gene is a common gene mutation in patients with nasopharyngeal carcinoma. Methylation abnormality in patients with nasopharyngeal carcinoma is another important pathway of inactivation of P16 gene, deserving attention.