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在发展光纤探针制备和探针与样品近场间距非光学控制等关键技术基础上 ,我们研制成能与倒置光学显微镜联合使用的扫描切变力 /近场光学显微镜 ,并具有反射和透射等工作模式以及能在溶液环境中工作。利用这套系统 ,获得了多种样品的表面形貌和近场光学图像以及细胞内的荧光光谱。本文将对该系统和有关实验结果作简要介绍
Based on the development of key technologies such as preparation of optical fiber probes and non-optical control of the near-field distance between the probe and the sample, we have developed a scanning shear force / near-field optical microscope that can be used in conjunction with an inverted optical microscope and has the advantages of reflection and transmission Working mode and working in solution environment. Using this system, surface topographies and near-field optical images of a variety of samples as well as intracellular fluorescence spectra were obtained. This article will introduce the system and related experimental results briefly