用fura-2荧光染料测定了静息时的酶解分离心室肌细胞内游离Ca~(2+)浓度,40mMKCI和5mM咖啡因都能使心室肌细胞内游离Ca~(2+)浓度增加,但KCI增加游离Ca~(2+)浓度需要细胞外液中存在Ca~(2+),而咖啡因则否,提示KCl增加细胞内游离Ca~(2+)浓度可能是通过细胞外Ca~(2+)内流,咖啡因则使细胞内贮存Ca~(2+)释放。 Fura-2 fluorescent dye was used to determine the concentration of free Ca 2+ in ventricular myocytes at rest. 40 mM KCl and 5 mM caffeine could increase the concentration of free Ca 2+ in ventricular myocytes, However, the increase of free Ca 2+ concentration in KCI requires the presence of Ca 2+ in the extracellular fluid, while CaCl 2 increases the intracellular free Ca 2+ concentration by KCl, which may be caused by extracellular Ca ~ (2+) influx, caffeine release intracellular storage of Ca ~ (2+).