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为了分析黄瓜 IPT 家族基因,以及黄瓜 IPT 基因 CsIPT 与果实发育的关系,以黄瓜单性结实自交系‘EC1’和非单性结实自交系‘8419s-1’为试材,应用荧光定量 PCR 技术,在分析不同器官中CsIPT 表达特征的基础上,寻找果实发育中的作用基因,进一步分析这些基因在果实发育过程中的表达特征。结果表明:8 个黄瓜 IPT 基因(CsIPT1 ～ CsIPT8)核苷酸相似性在 17.3% ～ 33.2%范围内,氨基酸长度在 280 ～ 504 AA 之间,具有共同的 ATP/GTP 结合功能域[(A,G)-X4-G-K-(S,T)],CsIPT4 氨基酸序列中包含有一个锌指基序(C-X2-C-X12,18-H-X5-H)类似序列;黄瓜幼果中 CsIPT3、CsIPT5、CsIPT7 和 CsIPT8表达水平较高;CsIPT1、CsIPT3、CsIPT4 和 CsIPT5 的表达量在未发育的果实中有增加的趋势,CsIPT2和 CsIPT8 在未发育的果实中表达基本无变化,而在授粉后发育的果实中表达量很高,说明这两个基因可能与授粉后黄瓜果实发育调控有关。此外,CsIPT2 在天然单性结实材料‘EC1’果实发育早期表达较高,推测其在黄瓜单性结实果实发育中发挥着重要作用。
In order to analyze the relationship between IPT family genes of cucumber and CsIPT of cucumber IPT gene and fruit development, the inbred lines ’EC1’ of cucumber and ’8419s-1’ of non-parthenocarpic inbred lines were used as materials, Technology, based on the analysis of CsIPT expression characteristics in different organs, looking for the role of genes in fruit development, and further analysis of the expression characteristics of these genes in the fruit development process. The results showed that the nucleotide similarity of IPT gene (CsIPT1 ~ CsIPT8) was in the range of 17.3% ~ 33.2%, and the amino acid length was between 280 ~ 504 AA, which shared a common ATP / GTP binding domain [(A, The sequence of zinc finger motif (C-X2-C-X12,18-H-X5-H) contained in CsIPT4 amino acid sequence was similar to that of CsIPT3 , CsIPT5, CsIPT7 and CsIPT8 were higher than that of the control. The expression of CsIPT1, CsIPT3, CsIPT4 and CsIPT5 showed an increasing trend in undeveloped fruits, and CsIPT2 and CsIPT8 showed no change in underdeveloped fruits. However, The high expression level of the fruit in development indicates that these two genes may be related to the regulation of fruit development after pollination. In addition, CsIPT2 was highly expressed in the early developmental stages of fruit, ’EC1’, a natural parthenocarpic material, suggesting that CsIPT2 plays an important role in the development of unisexual fruit development in cucumber.