从青菜的下胚轴和根原生质体培养获得大量的愈伤组织并形成根。比较了影响青菜下胚轴和根原生质体分离的因素。培养基、培养方式、激素、椰子水、芸苔素等对培养效果有不同程度的影响。探讨了与青菜原生质体培养褐化有关的一些因素。进行了多种分化试验 A large amount of callus was obtained from the hypocotyls and root protoplasts of cabbage and formed roots. The factors influencing the separation of hypocotyl and root protoplast of Chinese cabbage were compared. The culture medium, culture method, hormone, coconut water, brassinolide and so on have different degrees of influence on the culture effect. Some factors related to the browning of protoplast of cabbage were discussed. A variety of differentiation assays were performed