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目的：探讨喉癌的发生机理。方法：应用免疫组化ＬＳＡＢ法对３０例喉鳞状细胞癌人乳头瘤病毒（ＨＰＶ）感染、Ｌａｎｇｅｒｈａｎｓ细胞（ＬＣ）及ｐ５３蛋白表达进行了研究。结果：２６．７％的病例可以检测到ＨＰＶ抗原成分。ＨＰＶ感染的癌旁粘膜内ＬＣ数量明显少于无感染者，且形态也发生改变。ｐ５３蛋白表达阳性率在ＨＰＶ感染组（３７．５％）明显低于ＨＰＶ检测阴性组（８３．３３％）。结论：提示ＨＰＶ、ＬＣ、ｐ５３在喉癌发生发展过程中起一定作用，且相互影响，ＨＰＶ感染引起ＬＣ数量减少，局部免疫功能降低，ＨＰＶ感染还可能通过表达的肿瘤蛋白或其他机制使抑癌基因ｐ５３失活，进而导致肿瘤的发生。
Objective: To investigate the mechanism of laryngeal cancer. Methods: The expression of human papillomavirus (HPV), Langerhans cell (LC) and p53 protein in 30 cases of laryngeal squamous cell carcinoma were studied by immunohistochemical LSAB method. Results: HPV antigen components were detected in 26.7% of the cases. The number of LC in adjacent mucosa of HPV infection was significantly less than that in non-infected patients, and the morphology also changed. The positive rate of p53 protein expression in HPV infection group (37.5%) was significantly lower than HPV negative group (83.33%). Conclusions: The results suggest that HPV, LC and p53 play a role in the development of laryngeal carcinoma. The HPV infection causes a decrease in the number of LC and a decrease in the local immune function. HPV infection may also cause tumor suppression by expressing tumor proteins or other mechanisms Gene p53 inactivation, which led to the occurrence of tumors.