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Atlas~（TM）cDNA Expression array Ⅰ是Clontech公司1997年4月推出的第1张商品化大规模核酸阵谱,包括588个严格选择的处于严密转录调控、在不同生物过程中起关键作用的人类基因cDNA序列,分6个功能区（A,B,C,D,E,F）点阵于1张尼龙膜上。我们用其做了人胚鼻咽、成人鼻咽和鼻咽癌组织的基因表达谱,比较其差异。材料包括胚胎鼻咽2例,成人慢性鼻咽炎活检组织11例及鼻咽癌活检组织17例。提取总RNA,纯化Poly A+RNA,合成放射性标记cDNA探针,与阵谱杂交及放射自显影。结果见图1与表1。
Atlas ™ cDNA Expression Array Ⅰ is the first commercially available large-scale nucleic acid array published by Clontech in April 1997. It contains 588 strictly selected human genes that are under tight transcriptional regulation and play key roles in different biological processes Gene cDNA sequence, divided into six functional areas (A, B, C, D, E, F) dot matrix on a nylon membrane. We used it to make human embryo nasopharyngeal, adult nasopharyngeal and nasopharyngeal carcinoma gene expression profiles, compare the differences. Materials include 2 cases of embryonic nasopharyngeal, 11 cases of adult chronic nasopharyngitis biopsy and 17 cases of nasopharyngeal carcinoma biopsy. Total RNA was extracted, Poly A + RNA was purified, radiolabeled cDNA probes were synthesized, hybridized with the array and autoradiographed. The results shown in Figure 1 and Table 1.