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目的探讨肿瘤干细胞标志物CD133和CD166在结直肠癌中的表达及其临床意义。方法采用免疫组织化学方法检测102例结直肠癌患者的癌组织、40例结直肠癌患者的癌旁正常黏膜组织及40例健康人群的结肠腺瘤组织中CD133和CD166的表达,分析其与结直肠癌临床病理参数间的关系。结果 102例结直肠癌患者的癌组织中,CD133和CD166的阳性率分别为71.6%和60.8%,而对照组结肠腺瘤中的阳性染色率分别为17.5%和15.0%,正常黏膜组织阳性率均为0%,两两比较差异均具有统计学意义(P<0.05)。CD133和CD166蛋白的表达与结直肠癌的淋巴结转移和术后复发转移有相关性(P<0.05)。CD133和CD166双阳性组的5年生存率为42.5%,双阴性组为75.0%,差异具有统计学意义(P<0.05)。结论 CD133和CD166在结直肠癌癌组织中的表达高于腺瘤组织和癌旁组织,两者阳性组与预后呈负相关,提示其可能与结直肠癌的恶性演进有关,可作为结直肠癌侵袭、淋巴结转移和预后的重要标志。
Objective To investigate the expression of tumor stem cell markers CD133 and CD166 in colorectal cancer and its clinical significance. Methods Immunohistochemistry was used to detect the expression of CD133 and CD166 in 102 cases of colorectal cancer, 40 cases of colorectal cancer adjacent normal mucosa and 40 cases of colorectal adenoma. Relationship between clinical and pathological parameters of rectal cancer. Results The positive rates of CD133 and CD166 in cancer tissue of 102 patients with colorectal cancer were 71.6% and 60.8%, respectively. The positive staining rates of colorectal adenoma in control group were 17.5% and 15.0% respectively. The positive rate of normal mucosa All of them were 0%. There was a statistically significant difference between every two groups (P <0.05). The expressions of CD133 and CD166 proteins were correlated with lymph node metastasis and recurrence and metastasis of colorectal cancer (P <0.05). The 5-year survival rate was 42.5% in CD133 and CD166 double positive groups and 75.0% in double negative groups, with significant difference (P <0.05). Conclusions The expression of CD133 and CD166 in colorectal cancer tissues is higher than that in adenomas and paracancer tissues. The positive rates of CD133 and CD166 are negatively correlated with prognosis, suggesting that CD133 and CD166 may be associated with the malignant progression of colorectal cancer and may be used as colorectal cancer Invasion, lymph node metastasis and prognosis of the important signs.