论文部分内容阅读
TGF-β/Smad signaling pathway triggers diverse cellular responses among different cell types and environmental conditions.Quantitative analysis of protein-protein interactions involved in TGF-β/Smad signaling is demanded for understanding the molecular mechanism of this signaling pathway.Live-cell single-molecule microcopy with high spatiotemporal resolution is a new tool to monitor key molecular events in a real-time manner.In this review,we mainly presented the recent work on the quantitative characterization of TGF-β/Smad signaling proteins by singlemolecule method,and showed how it enabled us to obtain new insights about this canonical signaling process.