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[目的]为开展Spo A基因表达调控规律的研究及利用SopA启动子片段构建甘薯高效表达载体提供理论依据。[方法]应用PCR技术从徐薯18中克隆出甘薯贮藏蛋白A基因5′端1条长度为348 bp的DNA片段,然后应用PLACE、PlantCARE在线启动子预测工具进行分析。[结果]Spo A启动子序列除含有TATA-box、CAAT-box等启动子的保守元件外,还有蔗糖诱导响应元件CMSRE1S、P8作用位点等重要的顺式作用元件,以及一些其他调控序列如MYB结合位点。从序列分析结果可以推测该基因的启动子具有蔗糖创伤诱导响应的功能。[结论]该研究分析和推测了Spo A启动子序列中的作用调控元件,为今后利用该启动子构建甘薯高效表达载体奠定了基础。
[Objective] The research aimed to carry out the research on regulation regulation of Spo A gene expression and provide theoretical basis for constructing sweet potato high efficient expression vector by using SopA promoter fragment. [Method] A DNA fragment of 348 bp in length from the 5 ’end of sweet potato storage protein A gene was cloned by PCR from Xushu 18 and analyzed by PLACE and PlantCARE online promoter prediction tools. [Result] In addition to the conserved elements such as TATA-box, CAAT-box and other promoters, the Spo A promoter sequence also had important cis-acting elements such as sucrose-responsive response elements CMSRE1S and P8, as well as some other regulatory elements Such as MYB binding sites. From the results of sequence analysis, we can speculate that the promoter of this gene has the function of induction of sucrose trauma. [Conclusion] This study analyzed and deduced the regulatory elements in the promoter sequence of Spo A, which laid the foundation for the future construction of high efficient sweet potato expression vector.