用大肠癌HT29作抗原免疫小鼠,取其脾脏的淋巴细胞与骨髓瘤细胞融合,产生三株杂交瘤细胞——Ga_3、Gb_6和Cb-5,其所作用的抗原为肿瘤相关抗原。间接免疫荧光的研究发现:三株抗体与肿瘤的石腊切片无反应,但与冰冻切片及大肠癌细胞株作短期固定者反应阳性。Ga_3属IgG_(2a),Gb_6和Cb_5属IgM。三株抗体可能有助于结肠癌的免疫诊断。 The mice were immunized with the colorectal cancer HT29 as an antigen and lymphocytes from the spleen were fused with myeloma cells to produce three hybridoma cells, Ga3, Gb6, and Cb-5, and the antigen they used was a tumor-associated antigen. The indirect immunofluorescence study found that the three antibodies did not respond to the paraffin sections of the tumor, but had positive reactions with frozen sections and colon cancer cell lines for short-term fixation. Ga3 belongs to IgG_(2a), Gb_6 and Cb_5 belong to IgM. Three antibodies may contribute to the diagnosis of colon cancer.