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Q热立克次体Henzerling株鸡胚培养的感染卵黄囊在蔗糖-磷酸缓冲生理盐水中制成悬液,以差速离心法提取,继用复方泛影葡胺密度梯度离心法排除鸡胚卵黄囊成分,提取和纯化活的立克次体。在线性密度梯度液中段,立克次体形成乳白色界限明晰的区带。 经光学显微镜及电子显微镜检查,立克次体的形态完整。在豚鼠、小白鼠及鸡胚感染实验中表明仍保留着活力及感染性。免疫荧光试验,单克隆抗体保护试验及补体结合试验证明对Ⅰ相及Ⅱ相的抗原性无变化。此提纯的制剂中未查出污染的鸡胚卵黄囊成分,其平均回收量为0.868mg/g卵黄囊或0.3472mg/g卵黄囊。
Q Infected yolk sacs from Henzerling strain of Chick embryo were suspended in sucrose-phosphate buffered saline and extracted by differential centrifugation. The yolk sacs were excised from the egg yolk by compound diazepam dextran gradient centrifugation Capsule components, extract and purify live rickettsia. In the mid-section of the linear density gradient, rickettsia forms a clear-cut zone of opalescence. By optical microscopy and electron microscopy, rickettsia morphology is complete. In guinea pigs, mice and chick embryo infection experiments showed that still retain vitality and infectivity. Immunofluorescence assay, monoclonal antibody protection assay and complement fixation assay showed no change in the antigenicity of Phase I and Phase II. The contaminated chick embryo yolk sac fraction was not detected in this purified formulation with an average recovery of 0.868 mg / g yolk sac or 0.3472 mg / g yolk sac.