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采用热和静水压刺激草鱼受精卵诱导四倍体。在21C受精后36、39和42分钟,对受精卵进行9000和10000psi(磅/平方寸)静水压处理,鱼苗孵出后一天测定四倍体的诱导率为25%～100%(平均52.5%)。最适处理时间一般在卵裂前15～20分钟,这时能抑制核分裂。在受精后65、66和70分钟,对多细胞受精卵进行压力刺激(9000和10000psi),也可以诱导四倍体,受精后33～60分钟,对受精卵进行1.25和1.5分钟42C热刺激,四倍体的诱导率为0～100%;而同样温度的1分钟热刺激则不能产生四倍体。总之,大多数处理组的孵化率完全不同,且直接和四倍体转化有关。四倍体鱼苗存活天数没有超过50日龄。可以认为死亡鱼苗中有部分为非整倍体(次四倍体)和2n～4n嵌合体所引起。
Heat and hydrostatic stimulation of grass carp fertilized eggs to induce tetraploids. Fertilized eggs were hydrostatically treated at 9000 and 10000 psi 36, 39, and 42 minutes after fertilization at 21 C, with tetraploid induction at 25% -100% on day one hatching (mean 52.5 %). Optimal treatment time is generally 15 to 20 minutes before cleavage, then can inhibit nuclear fission. Stressful stimulation of multicellular zygotes (9000 and 10000 psi) at 65, 66 and 70 minutes after fertilization can also induce tetraploids, 33-60 minutes after fertilization, 42 ° C thermal stimulation of fertilized eggs at 1.25 and 1.5 minutes, Tetraploid induction rate of 0 to 100%; the same temperature for 1 minute thermal stimulation can not produce tetraploids. In conclusion, the hatching rates for most treatment groups are quite different and are directly related to tetraploid transformation. Tetraploid fry survival days did not exceed 50 days of age. It can be considered that some of the dead fry are caused by aneuploidy (subtetraloid) and 2n ~ 4n chimeras.