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为明确芦笋茎枯病菌的最适产孢条件,采用平板培养法研究了培养基、温度、光照和碳氮源等因素对其分生孢子器和分生孢子产生量的影响。结果表明,芦笋茎枯病菌在PDA、NLPDA和OLPDA等富营养培养基上分生孢子器和分生孢子的产生量均较大,其数量分别大于每皿10个和2.0×107个;而在WA和Czapec培养基上较小。在28℃下的分生孢子器和分生孢子产生量均较大,其数量分别大于每皿10个和5.0×107个;达到34℃后,其数量均为0。24 h黑光培养下分生孢子器和分生孢子的产生量均较大,其数量分别大于每皿40个和5.0×107个;完全黑暗下较小。供试碳源均未明显促进基础培养基产生分生孢子器和分生孢子,其数量接近0;酵母粉作为氮源时其产生量均较大,而硫酸铵作为氮源时其产生量接近0。研究表明,培养基、温度和光照对芦笋茎枯病菌产孢的影响较大,某些氮源可提高产孢量,而碳源对其产孢几乎无影响。
In order to determine the optimal spore forming conditions of Asparagus officinalis, the effects of medium, temperature, light and carbon and nitrogen sources on the production of conidia and conidia were studied by plate culture method. The results showed that asparagus stems and pathogens produced more conidia and conidia on eutrophic medium such as PDA, NLPDA and OLPDA, respectively, which were more than 10 and 2.0 × 107 per dish respectively. However, WA and Czapec medium smaller. At 28 ℃, the production of conidia and conidia were both larger than 10 and 5.0 × 107 per dish, respectively. After reaching 34 ℃, the numbers were all 0.24 h in black light culture The production of conidia and conidia were both larger than that of 40 and 5.0 × 107 per dish, respectively, and less in complete darkness. The test carbon source did not significantly promote the basic medium to produce conidia and conidia, the number of close to 0; yeast powder as a nitrogen source when the amount were large, and ammonium sulfate as a nitrogen source when the amount produced 0. The results showed that medium, temperature and light had a significant effect on the sporulation of Asparagus officinalis. Some of the nitrogen sources increased the amount of sporulation, while the carbon source had little effect on sporulation.