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背景:由于肝脏既是胰岛素的作用部位,又是相对的免疫特惠区,排斥反应小,肝窦及其小静脉结构还有利于胰岛的居留和生长,可供移植容积大,有利于胰岛的生存,因此肝脏是一个较为理想的移植部位。目的:建立一种经门静脉胰岛细胞肝内移植治疗SD大鼠1型糖尿病的动物模型。方法:采用文献方法制备SD大鼠胰岛细胞。以腹腔注射链尿佐菌素诱导建立SD大鼠1型糖尿病模型,随机数字表法分为2组:实验组经门静脉主干穿刺输注SD大鼠胰岛1000胰岛当量1.5mL;对照组经门静脉主干穿刺输注无血清1640液1.5mL。术后未给予免疫抑制剂,观察两组大鼠出血量、血糖、胰岛素水平及肝脏组织形态学变化。结果与结论:所有大鼠均移植成活,门静脉穿刺一次成功率90%,出血量<0.5mL。胰岛移植大鼠血糖降为正常的时间1～6(3.7±1.7)d,移植胰岛存活时间为2～15(8.4±4.1)d。术后2周内实验组大鼠空腹血清胰岛素水平明显高于对照组(P<0.05,P<0.01)。病理结果证实,移植大鼠肝细胞形态、肝小叶结构均正常,肝实质未见坏死灶,血管内无血栓形成;门静脉主干未见狭窄,亦未发生感染,说明胰岛细胞在肝窦内存活并能发挥功能。证实大鼠胰岛经门静脉主干穿刺输注肝内移植是胰岛移植基础研究的理想模型。
Background: The liver is not only the site of action of insulin, but also the relative immunocompetent area. The small rejection, the structure of hepatic sinus and its small veins are also conducive to the residence and growth of islets. It can be used for large transplant volume and is conducive to the survival of islets, Therefore, the liver is a more ideal site for transplantation. OBJECTIVE: To establish an animal model of intrahippocampal transplantation of portal vein islets for type 1 diabetes in SD rats. Methods: SD rat islet cells were prepared by literature method. The model of type 1 diabetes mellitus induced by intraperitoneal injection of streptozotocin in SD rats was established. The random number table was divided into two groups: the experimental group received 1.5 mL insulin islets equivalent to 1.5 mL in the islets of the SD rats via the portal vein puncture; Puncture infusion of serum-free 1640 solution 1.5mL. Immunosuppressive agents were not given after operation. The amount of blood loss, blood glucose, insulin level and liver histomorphology were observed in both groups. RESULTS AND CONCLUSION: All rats were transplanted alive. The success rate of portal vein puncture was 90% and the bleeding volume was less than 0.5mL. The blood glucose in islet transplantation rats decreased to normal ranged from 1 to 6 (3.7 ± 1.7) d, and the survival time of transplanted islets was 2 to 15 (8.4 ± 4.1) days. Fasting serum insulin level in experimental group was significantly higher than that in control group within 2 weeks after operation (P <0.05, P <0.01). Pathological results confirmed that the morphological changes of hepatic cells and the structure of hepatic lobules in normal rats were normal. There was no necrotic foci in hepatic parenchyma and no thrombosis in blood vessel. No stenosed portal vein and no infection occurred, which indicated that islet cells survived in hepatic sinusoids Can play a role. Confirmed that the transplanted intra-hepatic transplantation of rat islets via the portal vein is the ideal model for the basic research of islet transplantation.