通过实验发现１ ％ ＮＰ４０ 、１ ％ Ｔｒｉｔｏｎ Ｘ１００ 、１ ％Ｔｗｅｅｎ２０ 等非离子型去垢剂均可以裂解ＨＡＶ 带毒细胞，释放甲肝病毒，且不影响ＨＡＡｇ 的ＥＬＩＳＡ 检测，其中１ ％ ＴｒｉｔｏｎＸ１００ 的效果最好，与常规超声超离方法比较，无明显差异（Ｐ＞００５） ，可用于甲肝疫苗感染性滴度检测的实际工作中。同时经过正交分析，并结合工作实际，认为：采用４ 天龄细胞、不洗细胞面、病毒吸附１ 小时、培养２６ ～２８ 天、中途根据细胞状况换液１ 次、培养到期用１ ％ Ｔｒｉｔｏｎ Ｘ１００ 液来裂解细胞释放病毒的简化程序并不会影响甲肝疫苗感染性滴度最后的检测结果 The results showed that 1% NP40, 1% Triton X100, 1% Tween20 and other non-ionic detergents could lyse HAV infected cells and release Hepatitis A virus without influencing ELISA of HAAg, among which 1% TritonX100 was the best , No significant difference (P> 005) compared with the conventional ultrasonication method, which can be used in the actual work of hepatitis B vaccine titer detection. At the same time through orthogonal analysis, combined with the actual work, that: the use of 4-day-old cells, the cell surface is not washed, the virus adsorption for 1 hour, cultured for 26 to 28 days, halfway based on cell status fluid 1, The simplified procedure of Triton X100 liquid to lyse cells and release the virus did not affect the final test result of the infectious titer of hepatitis A vaccine